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Abstract
Endophytic bacteria are beneficial microorganisms living in the tis-sues system of various parts of a plant, including fruits, leaves, twigs, and roots. The bacteria are stated as forming colonies without caus-ing any damage to the plant. Thus, this study aimed at isolating endo-phytic bacterial from the leaves, barks, and fruits of Kasturi plant (Mangifera casturi), screening its endophytic bacteria, determining the potential of those endophytic bacteria, identifying 16S rRNA and analyzing potential growth of the bacteria. The isolated endophytic bacteria appeared to show potential activity against pathogenic bac-teria Propionibacterium acnes with disc-diffusion methods. Besides, the observations on bacterial activities showed isolate L2, S2 and F4 isolated from leaves, bark and fruits, respectively, as the most potent producers of antibacterial compounds. Technically, those activities were indicated by the formation of clear zones. Molecular identifica-tion was investigated by applying PCR amplification on 16S rRNA gene. Furthermore, the isolate L2 was identified as Enterobacter clo-acae with 99% sequence similarities; however, isolates S2 and F4 were identified as Escherichia coli. Therefore, these findings sug-gested that the identified strains would contribute to any further searches for new sources of anti-acne substances.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
Sogandi, S., & Riyanto, J. (2020). Investigating the anti-acne potential of endophytic bacterial extracts isolated from Mangifera casturi in indigenous South Borneo, Indonesia. Journal of Agriculture and Applied Biology, 1(2), 54-63. https://doi.org/10.11594/jaab.01.02.03
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Sutomo, S., Wahyuono, S., Rianto, S., & Yuswanto, A. (2014). Antioxidant activity assay of extracts and active fractions of kasturi fruit (Mangifera casturi Kosterm.) using 1, 1-diphenyl-2-picrylhydrazyl method. Journal of Natural Products, 7(1), 124–130. http://journalofnaturalproducts.com/Vol-ume7/17_Res_paper-16.pdf
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolution-ary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731–2739. https://doi.org/10.1093/mol-bev/msr121
Triandriani, W., Saputri, D. D., Suhendar, U., & Sogandi. (2020). Antioxidant activity of endophytic bacterial extract isolated from clove leaf (Syzygium aromati-cum L.). Journal of Agriculture and Applied Biology, 1(1), 9 – 17. https://doi.org/10.11594/jaab.01.01.02
Wong, W. R., Oliver, A. G., & Linington, R. G. (2012). De-velopment of antibiotic activity profile screening for the classification and discovery of natural prod-uct antibiotics. Chemistry & Biology, 19(11), 1483–1495. https://doi.org/10.1016/j.chem-biol.2012.09.014.
Akinsanya, M.A., Goh, J. K., Lim, S. P., & Ting, A. S. Y. (2015). Diversity, antimicrobial and antioxidant ac-tivities of culturable bacterial endophyte communi-ties in Aloe vera. FEMS Microbiology Letters, 362(23). https://doi.org/10.1093/femsle/fnv184
Alvin, A., Miller, K. I., & Neilan, B. A. (2014). Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. Microbio-logical Research, 169(7-8), 483–495. https://doi.org/10.1016/j.micres.2013.12.009
Brader, G., Compant, S., Mitter, B., Trognitz, F., & Ses-sitsch, A. (2014). Metabolic potential of endophytic bacteria. Current Opinion in Biotechnology, 27, 30–37. https://doi.org/10.1016/j.copbio.2013.09.012
Chabib, L., Muhtadi, W. K., Rizki, M. I., Rahman, R. A., Su-hendri, M. R., Hidayat, A. (2018). Potential medici-nal plants for improve the immune system from Borneo Island and the prospect to be developed as nanomedicine. MATEC Web of Conferences, 154(04006). https://doi.org/10.1051/matec-conf/201815404006
Cho, S. J. et al. (2002). Endophytic bacillus sp. Isolated from the interior of balloon flower root. Bioscience, Biotechnology and Biochemistry, 66(6), 1270–1275. https://doi.org/10.1271/bbb.66.1270
Dasari, T.R., Inamdar, S. M., & Pawar, K. V. (2015). Study on production of bioactive compounds and plant promoting ability of endophytes isolated from Rosa sp. and Mangifera indica. International Journal of Current Microbiology and Applied Sciences, 4(2), 136–143. http://www.ijcmas.com.
Fakhrudin, N., Hastuti, S., Andriani, A., Widyarini, S., & Nurrohmad, A. (2015). Study on the antiinflamma-tory activity of artocarpus altilis leaves extract in mice. International Journal of Pharmacognosy and Phytochemical Research, 7(6), 1080–1085. http://im-pactfactor.org/PDF/IJPPR/7/IJPPR,Vol7,Issue6,Arti-cle9.pdf
Fernández, J. (2019). Multidrug-resistant bacterial infec-tions in patients with decompensated cirrhosis and with acute-on-chronic liver failure in Europe. Jour-nal of hepatology, 70(3), 398–411. https://doi.org/10.1016/j.jhep.2018.10.027
Khan, Z. Z., & Moore, T. A. (2009). Recurrent epidural ab-scess caused by propionibacterium acnes. Kansas Journal of Medicine, 2(4), 92–95. https://jour-nals.ku.edu/kjm/article/download/11302/10709/
Lynn, D. D., Umari, T., Dunnick, C. A., & Dellavalle, R. P. (2016). The epidemiology of acne vulgaris in late adolescence. Adolescent Health, Medicine and Ther-apeutics, 7(1), 13–25. https://doi.org/10.2147/AHMT.S5832
Nurunnabi, T., Al-Majmaie, S., Nakouti, I., Nahar, L., Rah-man, S., Sohrab, M., Billah, M., Ismail, F., Sharples, G., & Sarker, S. D. (2018). Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala, a mangrove plant of the Sundarbans. Asian Pacific Journal of Tropical Medicine, 11(5), 350–354. https://doi.org/10.4103/1995-7645.233183
Sinnot, S. J., Bhate, K., Margolis, D.J., Langan, S. M. (2016). Antibiotics and acne: an emerging iceberg of antibi-otic resistance? British Journal of Dermatology, 175, 1127–1128. https://doi.org/10.1111/bjd.15129
Skroza, N., Tolino, E., Mambrin, A., Zuber, S., Balduzzi, V., Marchesiello, A., Bernardini, N., Proietti, I., & Po-tenza, C. (2018). Adult acne versus adolescent acne: a retrospective study of 1,167 patients. The Journal of clinical and aesthetic dermatology, 11(1), 21–25. https://pubmed.ncbi.nlm.nih.gov/29410726/
Sogandi., Fitrianingrum, M., & Thursina, A. (2020). Iden-tification of bioactive compound and antbacterial activity of noni fruit (Morinda citrifolia l) extract as inhibitor Propionibacterium acne. Buletin Penelitian Kesehatan, 48(1), 73–82. https://doi.org/10.22435/bpk.v48i1.2338
Sogandi., Mustopa, A. Z., Artika, I. M. (2019). The charac-terization of bacteriocins produced by Lactobacillus plantarum strains isolated from traditional fer-mented foods in Indonesia and the detection of its plantaricin-encoding genes. Indonesian Journal of Biotechnology, 24(1), 1–7. https://doi.org/10.22146/ijbiotech.42582
Sogandi., & Nilasari, P. (2019). Isolation and molecular identification of endophytic bacteria from Noni fruits (Morinda citrifolia l) and their antibacterial activity. IOP Conference Series: Earth Environmental Science, 299, 1–11. https://doi.org/10.1088/1755-1315/299/1/012020
Strobel, G., Ford, E., Worapong, J., Harper, J. K., Arif, A. M., Grant, D. M., Fung, P. C. W., & Ming, W. C. R. (2002). Isopestacin, an isobenzofuranone from Pestalo-tiopsis microspora, possessing antifungal and anti-oxidant activities. Phytochemistry, 60(2), 179–183. https://doi.org/10.1016/S0031-9422(02)00062-6
Suhendar, U., Fathurrahman, M., & Sogandi. (2019). Anti-bacterial activity and mechanism of action of meth-anol extract from kasturi mango fruit (Mangifera casturi) on caries-causing bacterium Streptococcus mutans. Jurnal Kimia Sains dan Aplikasi, 22(6), 235–241. https://doi.org/10.14710/jksa.22.6.235-241.
Sutomo, S., Wahyuono, S., Rianto, S., & Yuswanto, A. (2014). Antioxidant activity assay of extracts and active fractions of kasturi fruit (Mangifera casturi Kosterm.) using 1, 1-diphenyl-2-picrylhydrazyl method. Journal of Natural Products, 7(1), 124–130. http://journalofnaturalproducts.com/Vol-ume7/17_Res_paper-16.pdf
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolution-ary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731–2739. https://doi.org/10.1093/mol-bev/msr121
Triandriani, W., Saputri, D. D., Suhendar, U., & Sogandi. (2020). Antioxidant activity of endophytic bacterial extract isolated from clove leaf (Syzygium aromati-cum L.). Journal of Agriculture and Applied Biology, 1(1), 9 – 17. https://doi.org/10.11594/jaab.01.01.02
Wong, W. R., Oliver, A. G., & Linington, R. G. (2012). De-velopment of antibiotic activity profile screening for the classification and discovery of natural prod-uct antibiotics. Chemistry & Biology, 19(11), 1483–1495. https://doi.org/10.1016/j.chem-biol.2012.09.014.